Bologna harnessed the momentum from their surprise win at Inter Milan on Friday to beat Fiorentina 2-0 at home on Tuesday.
First-half goals from Alessandro Diamanti and Gaston Ramirez secured their second straight victory.
It also moved them up two places to 14th in the Serie A table, while Fiorentina dropped a place to 13th.
For Fiorentina it was a second straight reverse following their 3-0 home defeat by Napoli, also on Friday.
Bologna had stunned Inter 3-0 at the San Siro and proved that was no fluke with a result that moved them well clear of the drop zone.
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